Development and evaluation of in vivo engineered vascular grafts in a porcine model
TC Rothuizen, F. Damanik, J.F. Hamming, M.J.T. Visser, I.E. Hoefer, L. Moroni, T.J. Rabelink, J.I. Rotmans
Voorzitter(s): prof. dr. M.M.H. Kramer, VUmc, Amsterdam & dr. C.G. Vermeij, Deventer Ziekenhuis
Woensdag 22 april 2015
15:00 - 16:00u
in Zaal 0.5
Categorieën: parallelsessie (case reports/research)
Parallel sessie: Parallelsessie 4: Case reports/research
Background:
Vascular access remains the Achilles' heel of hemodialysis. Tissue engineered blood vessel (TEBV) may offer an alternative. Polymer rods were developed that upon implantation evoke an inflammatory response culminating in encapsulation by a fibrocellular capsule. We hypothesized that, after extrusion of the rod, the fibrocellular capsule differentiates into an adequate vascular conduit, after grafting into the vasculature.
Methods:
Per pig, 8 rods were implanted subcutaneously. After 4 weeks, rods with tissue capsules grown around it, were harvested. Per pig, 2 tissue capsules were grafted bilaterally as carotid artery interposition. One and 4-week later, patency was evaluated by angiography whereupon pigs were sacrificed and tissue capsule morphology was evaluated.
Results:
Rods were encapsulated by thick, well vascularized tissue capsules, mainly composed of circumferentially aligned fibroblasts, collagen and few leukocytes, with adequate mechanical strength. Patency was 100% after 1week and 87,5% after 4weeks. Time to hemostasis after cannulation with a dialysis needle was
Conclusion:
Autologous TEBVs were created with sufficient mechanical strength enabling autologous vascular grafting with a 4week-patency of 87,5%. Future studies should evaluate its potential as arteriovenous-graft.
